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normal human osteoblasts  (ATCC)


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    ATCC normal human osteoblasts
    Normal Human Osteoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human osteoblasts/product/ATCC
    Average 98 stars, based on 936 article reviews
    normal human osteoblasts - by Bioz Stars, 2026-03
    98/100 stars

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    ATCC normal human fetal osteoblast cell line
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
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    Lonza cryopreserved cloneticstm normal human osteoblasts (nhost)
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
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    Lonza cloneticstm normal human osteoblasts (nhost)
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
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    ATCC normal human primary osteoblast hfob1 19 cells
    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 <t>osteoblasts.</t> ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).
    Normal Human Primary Osteoblast Hfob1 19 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Journal: Biomedicines

    Article Title: CAPE Derivatives as Potent Agents for Induction of Osteogenic Differentiation in DPSCs and Biomaterial Development

    doi: 10.3390/biomedicines13123039

    Figure Lengend Snippet: Cytotoxicity assessment of biomaterials enriched with CAPE derivatives on hFOB 1.19 osteoblasts. ( a ) MTT assay conducted by using 24 h extracts of the biomaterials after 48 h cell culture (PS medium—polystyrene extract served as negative control of cytotoxicity; * statistically significant results compared to PS medium, # statistically significant results compared to mat_control, $ statistically significant results compared to mat_ 1d _L, ^ statistically significant results compared to mat_ 1a _L, p < 0.05, One-way ANOVA followed by Tukey’s test); ( b ) confocal laser scanning microscope images showing live/dead staining of cells cultured on the surface of biomaterials for 48 h (Nomarski contrast was applied to show biomaterial microstructure; viable cells—green fluorescence; dead cells—red fluorescence; magnified 100×, scale bar = 200 µm).

    Article Snippet: Normal human fetal osteoblast cell line (hFOB 1.19, ATCC CRL-11372) was cultured in a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 Medium, supplemented with 2.5 mM L-glutamine, 0.3 mg/mL G418 (Sigma-Aldrich Chemicals, Warsaw, Poland), and 10% FBS (Pan-Biotech GmbH, Aidenbach, Bavaria, Germany) and maintained at 34 °C (5% CO 2 in air atmosphere).

    Techniques: MTT Assay, Cell Culture, Negative Control, Control, Laser-Scanning Microscopy, Staining, Fluorescence